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Disease Review: Dollar Spot

August 19, 2009

The time for the second application of fungicides on the new ​bentgrass research at the ISU horticulture research station has arrived. Chris Blume and I applied the curative, second treatment of Emerald® (boscalid) and Daconil® (chlorothalonil) today, August 18, 2009. The new bentgrass research is focusing on the susceptibility of different cultivars of bentgrass to the disease dollar spot. In the study, each plot is split, one side receiving no fungicides, while the other is treated with both a preventative and a curative application of boscalid and chlorothalonil. This year has proven to be an excellent year for dollar spot out at the research station and many of our bentgrass areas are spotted with the disease.

 

The disease dollar spot is caused by the fungal pathogen Sclerotinia homoeocarpa. This disease commonly infects many of our cool-season grasses. Dollar spot is a particular concern on bent/poa greens where the sunken pockets may interfere with putting quality. Dollar spot can develop over a wide range of temperatures (55-80°F) when the dew persists for long periods of time (longer than 8 hours). Dollar spot is also considered a low nitrogen disease and is most severe on nitrogen deficient stands of turf.

The pathogen causes blighted, circular patches of turf which are similar in size to a silver dollar (rarely larger than 2 in. in diameter) on low mown areas, and up to 6 in. or more in diameter on taller mown areas. The blighted spots of turf often occur in clusters and may merge together to produce larger blighted areas as disease development progresses. Active dollar spot infections produce a cottony white mycelia mass that is often evident on the turf during the early morning hours. When viewed under a microscope the mycelium will exhibit y-shaped branching and the presence of septum unlike the mycelia of pythium that are void of septum. In addition to the mycelia mass that may be visible, this pathogen also causes leaf lesions that have light tan centers and a red-brown margin. The leaf lesions of dollar spot are more commonly observed on taller turf species, such as Kentucky bluegrass, where the lesions take on the shape of an hour glass.

There are many ways to control dollar spot. One important management technique is to implement a satisfactory nitrogen fertility program. Nitrogen deficient stands of turf are more susceptible to dollar spot outbreaks. However, over stimulating with nitrogen may increase disease pressure from other, less desirable, diseases. Another option in control is to choose a less susceptible cultivar. Although there are no completely resistant cultivars of bentgrass, there are cultivars that are more resistant than others. For example, the cultivar Crenshaw® is far more susceptible to dollar spot than the cultivar Declaration®. Irrigation practices that limit the duration of leaf wetness will also help limit the occurrence of the disease. There are also fungicides available for the control of dollar spot. These fungicides often require multiple applications to effectively control this disease. Also, fungicides that are classified as site specific inhibitors have a high risk of resistance development and their repetitive use should be avoided. Some effective fungicides are Emerald®, Daconil®, Curlan®, and Tourney® along with many others.

Nick Dunlap

 

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Research Update: Keith Rincker, KRincker@cdga.org

October 16, 2009

North Central Region Collaborative Effort...and the data says

Dollar spot was the talk for the last two weeks of September, but now the night temps will hold off any development except for a possible warm spell left in the year. Now is the time to look back and evaluate our dollar spot information. We had a chance to evaluate our new fairway bentgrass variety trial during the highest disease pressure of the season. The borders of ‘Alpha’ and several entries were lighting up! It has been a long road for this trial. Twenty nine bentgrass
varieties were seeded last year and then reseeded this May. Now we have lowered the height (0.75 inches) slowly over the summer and finally we got some disease.

Next year we will learn (again)
Next year we will get a better idea for disease resistance of these varieties. So far our variation is still large and another years worth of data will be needed to determine the genetic resistance in the new varieties like ‘CY-2', ‘Kingpin’, ‘Shark’, and ‘007’. This data does show that varieties have been released with comparable resistance to ‘L-93’. Hopefully with more time we can say that there are a handful of varieties with greater resistance than ‘L-93’. In National Turfgrass Evaluation Program (NTEP) trials, ‘Declaration’ has shown promise. ‘Alister’ is actually a colonial bentgrass species. In previous research on Sunshine Course, colonial varieties are damaged by brown patch and weeds become a problem.

The “take home message”...
The goal of this research conducted here in Lemont and 11 other Midwestern universities is to determine how many fungicide sprays are needed when varieties with greater resistance are used.

The L-93 Story at North Shore
This year at North Shore Country Club we set out treatments on a fairway strip of ‘L-93’. Only one application was made on May 14 and the plan was to see which treatments provided the longest control. Bayleton, Emerald, Daconil, Banner Maxx, and Chipco GT were all applied and we waited for dollar spot. Mid June came around, and no dollar spot – too cold. The beginning of July came and only a tiny bit of dollar spot. The beginning of August came around and finally dollar spot reached 1-3%in all plots. Fungicide or not, all treatments were the same for percent dollar spot incidence and plot visual quality.

No differences...
We found no differences in our statistics. What did we learn? Maybe May 14 is too early for dollar spot control. Maybe we can reduce our fungicide inputs by utilizing genetic resistance and eliminating one or two applications early in the summer. Our heads are already spinning around to design research in 2010.

Emerald for next year’s research...
So far our thoughts are to pick one fungicide and apply on selected dates from May through June or July on each ‘L-93’ and a bentgrass/Poa mixture. This will give us an optimal timing for first application to control dollar spot on two different turfgrasses. For now we will write down our thoughts and continue to analyze the research.

Keith Rincker
KRincker@cdga.org
Chicago District Golf Association
11855 Archer Avenue
Lemont, IL 60439

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The Year of Brown Patch, Tim Sibicky, CDGA

July 21, 2010

Above normal daytime temperatures of 90+ degrees have been accumulating quickly (now up to 11 days in Lemont, IL), and the combination of high nighttime temps and high dew points has created a precarious situation for the rapid appearance of brown patch. This soilborne fungal disease is caused by the pathogen Rhizoctonia solani. Recent weather has been fairly dry since July 4th, and although it has been much needed for superintendents, it has added further
environmental stress to any previously weakened turfgrass plants. Damage by Rhizoctonia is now obvious (Figure 1).

Damage by Rhizoctonia

Research trials on Sunshine Golf Course have been providing excellent data on product testing for dollar spot. In addition, the exceptionally conducive environmental conditions for development of brown patch have allowed us to monitor effects of the products on both diseases. Untreated plots in all replications on July 15 showed greatest disease pressures at 40% brown patch and 15% dollar spot. Visible symptoms for brown patch were most severe on untreated plots as expected (Figure 2).

Visible Brown Spot

Of fungicides, the 21 day interval of the Emerald treatment at 0.18 oz had highest brown patch visibility at 20%, which when compared to the control was still far less. Other treatments that showed amounts of brown patch were Chipco26GT (dicarboximide family), Insignia (QoI), and the 14d low rate of Reserve (chlorothalonil + DMI). The fungicides that performed well with no visible symptoms of brown patch disease have been Daconil Ultrex (chlorothalonil), Honor (QoI
+ carboximide), Concert (chlorothalonil + DMI), Insignia, Renown (chlorothalonil + QoI) and Reserve (chlorothalonil + DMI) at both the 21d and the 14d high rates.

Brown Patch Disease

Treatments that have provided the best dollar spot disease control so far include: Daconil Ultrex, Chipco26GT, Heritage + Daconil, Insignia, Concert and Reserve at 21d (high rate). Treatments of Emerald, Honor, Renown and Reserve at 14d (high and low rates) all provided good levels of disease control. All fungicide treatments provided acceptable levels of quality except for Concert, appearing off-color (due to the DMI-propizole) and with coarser visible leaves.


 

Tim Sibicky
TSibicky@cdga.org
Chicago District Golf Association
11855 Archer Avenue
Lemont, IL 60439

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The Floods of 2010

August 13, 2010

This is a week that people in Central Iowa will not forget for a long time. Ames received around 10 inches of rain in a three day period and the city experienced arguably its most devastating flood ever. The Squaw Creek and Skunk River are the two major waterways that run through town. Squaw Creek crested at 18.13 feet (flood stage is 9) which was just shy of the record crest of 18.5 experienced back in 1993. The Skunk River crested at 26.52 feet (flood stage is 20) which was above the 1993 crest of 25.53 feet. For those familiar with Ames, the picture above is a shot of Hilton Coliseum inundated in flood waters.

The majority of the flood waters have now receded and cleanup efforts are well underway. Trying to predict the survival of turfgrass in flooded conditions is tricky business and depends on so many factors. Weather is a big factor and has not been on our side so far this week. Temperatures have been in the low 90’s with the humidity pushing heat indices into the 100’s.

We were greeted to a healthy dose of mycelium at our research station this morning. During my time spend in industry, I learned that trying to visually distinguish between dollar spot and pythium blight is very difficult (at least it is for me!) so we brought the sample to campus for a closer look. Inspection of the sample under a compound microscope confirmed that the disease we were seeing was in fact Pythium blight.
 

 

We also stumbled upon some slime mold. Earlier in the year we had a post about slime mold on green height creeping bentgrass. This slime mold appeared on rough height Kentucky bluegrass. Remember that slime molds are more of an oddity, they’re unsightly but they are not considered harmful and control measures are not necessary.

I’ll leave you with a video courtesy of KCCI News Channel 8 showing an aerial view of the city this past Wednesday morning.

https://www.youtube.com/watch?v=YQSnfvRw8mU

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Dollar Spot Disease 2010, Tim Sibicky, CDGA Turfgrass Research Manager

September 30, 2010

Warmer temperatures this week have provided suitable environmental conditions for dollar spot (Sclerotinia homoeoecarpa) development on fairways. We are now beginning to see a late season surge in damage as we enter the autumn months. Thinking back to the beginning of the 2010 season, we set out to investigate the effectiveness of early season dollar spot programs and if you look (Figure 1) we are able to see the progression of the disease at a variety of different locations surrounding Chicago. So I ask, was it worth it to spray early in April-May? At our locations for this year’s Biorational study, ranging from North Shore Country Club in Glenview, IL Coyote Run Golf Course in Flossmor, IL and Briar Ridge CC in northwest Indiana, it is evident that the disease failed to take off until the middle of summer.
 

As an additional note: each of our locations vary in turfgrass composition with Coyote Run having a blend of Southshore and L93, Briar Ridge CC with Penncross and North Shore CC having a mix of creeping bentgrass and Poa annua. We tested seven treatments, Rhapsody 10 fl oz, Ecoguard 20 fl oz, Dew Cure 4.0 fl oz, Urea 0.15 lbs, Daconil 3.2 oz, Daconil 3.2 oz curative (as needed using 5% damage threshold).

Treatments are being applied at 14 day intervals at label rates and the plots at all locations are scouted weekly. If disease infection centers exceed an average of 5% area affected a curative application of 3.2 oz of Daconil is applied over the biorational treatment. We are able to understand the effectiveness of the different treatments by comparing visual quality, percentage of disease, and number of curative fungicide applications. Our goal is to reduce fungicide use and maintain turfgrass quality at levels required for fairways.

Results (Table 1). At Coyote Run GC, we have been able to maintain the Dew Cure treatment below the 5% threshold without applying a single curative application of Daconil! At this location we were also able to see a recuperative effect within the Urea treatment, only requiring a single application. At North Shore Country Club we were able to reduce the curative applications by two when using DewCure. All other plots required the same amount of curatives, four apps. At Briar Ridge with DewCure, we were able to reduce the number of curative required by one. At Briar Ridge we did observe phytotoxicity by DewCure. On August 10 an application of all products were made at a time when temperatures were 90+ degrees and turf was entering drought stress. This resulted in severe injury by DewCure alone.

These biorational products may play more important roles in disease management programs as we continue to progress into a reduced input future. As we continue to learn and understand the benefits and limitations, superintendents will be able to make better and more informed decisions. We may as well investigate alternatives now before we run out of options.

The final picture shows a plot treated with Dew Cure resisting dollar spot without receiving any curative applications during the summer of 2010 at Coyote Run GC.

Tim Sibicky
TSibicky@cdga.org
Chicago District Golf Association
11855 Archer Avenue
Lemont, IL 60439

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Holy Mycelium!

July 8, 2011

When I arrived to work this morning I was greeted by an outbreak of dollar spot. We had been on the dry side since the last week June but we received 1.33 inches of rain yesterday. The abundant rainfall coupled with the warm overnight temperatures, and high humidity led to conditions that were very conducive for dollar spot. Mycelium was especially abundant in our untreated creeping bentgrass areas.

Below are a few pictures from one of our creeping bentgrass fairway trials. This particular trial has 24 different cultivars of creeping bentgrass. Each plot is split in half and is either untreated or receives applications of Daconil and Emerald.

The objective of the trial is the determine the susceptibility of creeping bentgrass cultivars to dollar spot when maintained under reduced fungicide applications. Applications of the Daconil and Emerald mixture are scheduled based on a threshold of dollar spot severity in a cultivar with a high level of dollar spot resistance. Declaration is the indicator cultivar in this trial.

This picture shows the Declaration plot in one of the replications. The left half of the plot is the treated side and before today had only received one application on June 6. Declaration is a recently developed cultivar of creeping bentgrass that has relatively high resistance to dollar spot compared with other cultivars. Even the untreated side (right side) is holding up fairly well without receiving any fungicide to date.

This next picture shows a Penn A-4 plot. Here the right half of the plot is the treated side. As you can see, there are noticeable differences between cultivars of creeping bentgrass with respect to their disease resistance.

This trial along with many others will be on display during the Iowa Turfgrass Institute/Iowa State University Field Day on July 21. There is still time to register for the event.

Marcus Jones
Assistant Scientist

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Dollar Spot and Black Cutworms

July 11, 2014

Over the last three to four weeks I have received pictures from Neric Smith at Indian Hills CC (seen below), and also noticed dollar spot on several creeping bentgrass golf course tees/fairways across the state. Dollar spot is caused by the fungal pathogen Sclerotinia homoeocarpa. Generally, dollar spot develops when dew persists on the plant for greater than 8 hours. It is less dependent on temperature, and can develop over a wide range of temperatures (55-80°F). Turf deficient in nitrogen is the most likely situation where signs of dollar spot will occur. The disease gets its name from the silver-dollar-sized blighted areas that appear on closely mown creeping bentgrass. In Kentucky bluegrass and higher cut turf, the blighted area is about the size of a softball. Upon further examination of the turf affected by this disease, you will see an hour-glass shaped lesion in the middle of the leaf blade. The lesion is tan in the middle, surrounded by brown to orange halos on each side.

Active dollar spot infections produce a cottony white mycelium mass that is often evident on the turf during the early morning hours, not to be confused with spider webs. When viewed under a microscope the mycelium will exhibit y-shaped branching and the presence of septum (crosswalls). Pythium also produces this cottony white mass of mycelium but does not contain septum.

There are many ways to control dollar spot. The most important management technique is to increase N fertility in situations where N is found to be deficient. In addition to adding nitrogen; moderating thatch, removing dew, and preventing drought stress will help prevent the occurrence of dollar spot. The risk with many site-specific inhibitors is the development of dollar spot resistance. To prevent fungicide resistance use a rotation of chemicals and avoid repetitive use.  

The two pictures below were taken by Neric at Indian Hills.

 Cutworms:

The black cutworm is the most widely distributed cutworm that attacks turf in the United States. Cutworms do not overwinter in Iowa, but are brought up by winds from the south. Once they are established in the spring, they can undergo several life cycles before dying out in the fall. The adult is a dark-colored moth that is often observed fluttering around lights in the summer months. 

Cutworms, as the ones seen below, are commonly found in open aerification holes on golf course greens. A close look at the surface will reveal a lot of feeding damage around the holes.  They are often empty because of the feeding of birds. Cutworms are surface feeders and can easily be controlled with insecticides, the most common of these being Sevin (Carbaryl). While the larvae mainly emerge at night to feed, some may be seen on the putting surface during the day time, as was the case with the pictures below that was seen at Cedar Pointe in Boone a few weeks ago. 

 

 

 

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Name Brand vs. Generic Fungicides

January 4, 2010

This summer at Des Moines Golf and Country Club, Director of Grounds, Rick Tegtmeier, CGCS and two ISU Interns conducted a fungicide study that compared name brand versus generic products for dollar spot control. The two interns from Iowa State University were, Tyler Boley and Tayler Riggen. They worked closely with Luke Dant from Syngenta Professional Products to set up a replicated study on the golf course.

How they did it
Plots were arranged on fairways at two different locations on the course in order to account for spatial variability. The fairways contain ‘Penneagle’ creeping bentgrass maintained at 0.5 inches. One set of plots were located on the north course and the other on the south course. Each set of plots was replicated three times. Treatments included an untreated control, Daconil WeatherStik, Manicure 6FL, and Echo 6F ETQ fungicides. All products were applied at a rate of 2 oz/1000 ft2, to deliver the same amount of active ingredient, using a backpack sprayer with #8002 flat fan nozzles. Carrier volume was 44 gallons/acre. Spray applications began on May 14 and concluded August 13. Applications were made on 14-day intervals for a total of 7 applications. Percentage of turf affected by dollar spot was visually estimated on July 1 and August 14.

What they found
Each plot in the study succumbed to some dollar spot, but it was clear there were differences between the products in our study (Table 1).

Similar trends were evident on each of the test plots between the north and south courses but the disease pressure on the north course was far more severe. An application of Bayleton was made to the plots on the north course on June 22 to help reduce disease pressure. The main findings from the study are summarized below.

- Control plots sustained significant damage and possibly needed reestablishment.

- All chemicals succumbed to some breakthrough if applied on 14-day intervals. At the rate used in our study, shorter intervals are needed with all products.

- Manicure 6FL seemed to have good control until approximately 10 days after each application. After that, there was significant breakthrough.

- Echo ETQ had less than desired control through the 14-day period between applications. Echo ETQ fungicide is green in color which is designed to help cool the turf and in turn help prevent disease. In our opinion, the green colorant has little effect and was barely noticeable after being sprayed.

- Daconil WeatherStik had acceptable control until 13 and 14 days after application, and then some small breakthrough was evident. Recovery was not exceptional, but due to better initial control of dollar spot, Daconil provided the best control of dollar spot.

- One option would be to compare prices of products to see if Manicure 6FL or Echo ETQ could be applied on 10 day intervals for lesser overall price, however; this would increase labor costs and wear and tear on spray equipment, possibly negating any cost savings by purchasing these generic products. In our study, Manicure and Echo provided nearly the same level of dollar spot control.

 

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RUST, LEAF SPOT, AND DOLLAR SPOT

March 13, 2014

I am currently teaching the advanced turf course, Hort 451, on the web.  A part of that course, each student completes a paper or project.  Mary Broadfoot, a senior in Animal Ecology, chose to do 9 extension type bulletins.  Three of these are on turf diseases, three are on insects, and three are on weeds.

These tuned out so well that I have decided to share them on the blog site.  I will include them three at a time over the next few days.  The first three are on diseases.  She chose Rust, Leaf Spot, and Dollar Spot.

They are attached as pdf files below.  You can get the full text by clicking on the individual words below.  Several of the pictures used are from the internet.  She has sited each location from which information was used.

 Rust

https://drive.google.com/file/d/0BzSX2eos6s6hbTVHTEMxUktYREE/edit?usp=sh...

 Leaf Spot

https://drive.google.com/file/d/0BzSX2eos6s6hMkNVaHZBV19tY0k/edit?usp=sh...

Dollar Spot

https://drive.google.com/file/d/0BzSX2eos6s6hMGMxV3UxcTY0SFE/edit?usp=sh...

 

 

 

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2010 Creeping Bentgrass-Dollar Spot Study

June 1, 2011

This is the first of the 2011 research reports. It includes the 2010 results from our trial on creeping bentgrasses maintained at both fairway and green height. Tables 3 and 4 are jpg photos and you will need to click on them to be able to read them.

 

Creeping Bentgrass Dollar Spot Study

Christopher J. Blume and Nick E. Christians

 

Objectives

The objectives of this study are to determine the susceptibility of creeping bentgrass (Agrostis stolonifera) cultivars to dollar spot (Sclerotinia homeocarpa) under both green height and fairway height.

 

Materials and Methods

This fairway height (0.5”) and green height (0.25”) studies are being conducted at the Iowa State University Turfgrass Research Station it is part of a regional project being conducted at several of the Midwestern Universities. Both studies included 24 cultivars, although some of the cultivars differed on the two sites (Table 1). The green-height study area was established on a sand-capped area, and the fairway-height area was established on a native soil area (Nicollet clay-loam).

Both studies were established 17 September, 2008. The plots were allowed to mature until the spring of 2009. The plots were then split into untreated and fungicide treated halves. The study was conducted as a randomized split block design, with three replications.

Fungicide treatment timing was based on the cultivar ‘Declaration’, which is the most dollar spot tolerant cultivar in the study. Treatments were made to the green height plots when ‘Declaration’ was observed to have at least 5% of the plot area infested with dollar spot. The fairway height study area was treated when ‘Declaration’ was observed to have at least 10% of the plot area infested.

The fungicide mixture consisted of Emerald (0.18 oz product/1000ft2) and Daconil Ultrex (3.2 oz product/1000ft2), applied in 2 gallons water/1000ft2. The applications were applied using a modified spray boom, with two TeeJet XR flat fan nozzles. In 2010, three applications were made 24 June, 19 August, and 4 October.

 

Results

‘Memorial’ and ‘Penncross’, and ‘LS-44’ had the best quality ratings in the green height study in 2010, whereas ‘Allister’ received the lowest rating. In the fairway height study, ‘Alpha’, ‘LS-44’, and ‘Memorial’ were the highest rated cultivars, and ‘Independence’, ‘Declaration’ and ‘SR 1150’ received the lowest quality ratings (Table 2).

‘Century’ and ‘Imperial’ showed the most damage from dollar spot in both September and October in the green height study (Table 3). ‘Memorial’, ‘Declaration’, and ‘Crystal Bluelinks’ had the least damage in untreated plots at the end of the season.

There was not as much dollar spot in 2010 as there was in 2009 on the fairway height bentgrass. At the first two ratings of the season, there was more dollar spot in the treated side than in the untreated side of the plot. These plots had not been treated since the fall before. The reason for this observation is unknown. In August, no dollar spot was observed on treated or on untreated sides of the plot. In September, ‘Southshore’ and ‘Century’ had the most dollar spot on the untreated side of the plots, whereas ‘Memorial’, ‘Independence’, and ‘Declaration’ had the least dollar spot. In October, ‘Crenshaw’ and ‘Century’ had the most dollar spot and ‘Memorial’, ‘Alister’, ‘Pennlinks II’ and ‘Memorial’ had the least (Table 4).

 

Table 1. Varieties of creeping bentgrass in commercial bentgrass demonstration trial.

Entry No.

Fairway Height Varieties

Green Height Varieties

1

L-93

L-93

2

T-1

T-1

3

Alpha

Alpha

4

Putter

Putter

5

Southshore

Southshore

6

Kingpin

Kingpin

7

Crenshaw

Crenshaw

8

Imperial

Imperial

9

Century

Century

10

Penncross

Penncross

11

A-4

A-4

12

Crystal bluelinks

Crystal bluelinks

13

Alister

Penn A-1

14

Pennlinks II

Penn G-6

15

007

007

16

MacKenzie

MacKenzie

17

Tyee

Tyee

18

SR 1150

SR 1150

19

Memorial

Memorial

20

Independence

Independence

21

Declaration

Declaration

22

LS - 44

LS - 44

23

Bengal

Bengal

24

Penn G-6

Alister

 

 

 

Table 2. Quality ratings for green-height and fairway-height cultivars of the creeping bentgrass demonstration trial.

 

Quality

 

Quality

Cultivars (green)

2010

Cultivars (fairway)

2010

L-93

5.9β

L-93

6.4β

T-1

5.4

T-1

6.2

Alpha

5.4

Alpha

6.6

Putter

5.8

Putter

6.2

Southshore

5.8

Southshore

6.0

Kingpin

5.7

Kingpin

6.3

Crenshaw

6.2

Crenshaw

5.9

Imperial

5.6

Imperial

6.3

Century

5.5

Century

5.9

Penncross

6.2

Penncross

6.0

A-4

5.6

A-4

6.0

Crystal bluelinks

6.1

Crystal bluelinks

6.4

Penn A-1

5.9

Alister

6.1

Penn G-6

6.1

Pennlinks II

6.1

007

5.1

007

6.2

MacKenzie

5.3

MacKenzie

6.2

Tyee

5.0

Tyee

6.0

SR 1150

4.7

SR 1150

5.8

Memorial

6.3

Memorial

6.5

Independence

5.1

Independence

5.6

Declaration

5.0

Declaration

5.7

LS-44

6.2

LS-44

6.5

Bengal

5.7

Bengal

6.4

Alister

4.2

Penn G-6

6.4

LSD(0.05)

0.9

LSD(0.05)

0.6

Quality data rated on a scale of 9-1, with 9 = excellent turf; 1 = poorest quality; 6 = minimally acceptable.

Ratings are the average of three replications, averaged over the months of June to October.

Ratings observed only on fungicide-treated side of plot area.

βRatings are the average of three replications, averaged over the months of May to October.

 

 

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