Oxidative Stabilities of Soybean Oils that Lack Lipoxygenases

J. Am. Oil Chem. Soc. 73(10):1327-1336.
Shen, N., W. Fehr. L. Johnson, and P. White. 1996.

Lipoxygenase (LOX)-null soybean lines that lack LOX 2, or LOX 2 and 3, and contain normal (8.0-8.6%) or low (2.0-2.8%) linolenate (18:3) amounts were evaluated for their oil qualities and storage stabilities. Soybean oils of six genotypes were extracted by both laboratory- scale and pilot-plant systems and were refined, bleached, and deodorized in the laboratory. Citric acid was added to oils during the cool-down stage of deodorization. Two replications, separated at the point of conditioning, were evaluated for each genotype. Under storage conditions of 55-60°C in the dark, soybean oils with low 18:3 contents were significantly (P=0.05) more stable as measured by peroxide values than were oils with normal 18:3 contents, regardless of the LOX content of the beans. The volatile analysis showed few differences between oils with low and high 18:3 contents or among oils from bean that lack different LOX enzymes. After 16 d of storage, the amount of 1-octen-3-ol than did the oils that lacked LOX enzymes. Storage at 35°C under light showed no differences in volatile amounts or sensory evaluations after 14 d of storage. During storage, peroxide values tended to be lower in oils from beans with normal 18:3 content and in oils from beans with normal LOX content. Generally, the absence of LOX 2 or LOX 2 and 3, although having a small effect on lipid oxidation, was not as important to oil quality as was the 18:3 content.